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. 2008 Jun 20;283(25):17615–17623. doi: 10.1074/jbc.M709844200

FIGURE 7.

FIGURE 7.

Amino acid alignments of reqIgG subclasses and reqIgG7 mutants showing only CH2 and CH3 residues critical for interaction with protein A and protein G. Residue numbers (shown above sequences) correspond to human IgG1 numbering. In the corresponding hIgG1 sequence (shown for comparison at the top), residues implicated in interactions are indicated as follows: protein G, normal text; protein A, underlined; both protein A and protein G, box. The corresponding sequence for human IgG3 allotype IGHG3*01 (hIgG3*01) is also shown. In the middle section, the FgBP-binding subclasses eqIgG4 and eqIgG7 are shown above the non-binding subclasses. reqIgG7 mutants are shown in the lower part of the figure, with substitutions encircled. Ka values for interaction with FgBP1 are shown on the right. TLTD, too low to detect.