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Effect of RPA on AID activity on transcribed closed circular dsDNA substrate AID deamination activity was measured using a covalently closed circular dsDNA M13mp2T7 substrate, undergoing active transcription by T7 RNA polymerase (see Ref. 11).
| Mutation frequency (× 10–3) | |
|---|---|
| No AID | 0.8 ± 0.3 |
| WT AID | 3.7 ± 0.5 |
| WT AID + RPA (60 ng) | 4.7 ± 0.9 |
| WT AID + RPA (300 ng) | 2.5 ± 0.5 |
| WT AID + RPA (1 μg) | 2.2 ± 0.4 |
