Skip to main content
. 2008 May 16;9(6):569–575. doi: 10.1038/embor.2008.72

Figure 3.

Figure 3

LARP7 is a component of the 7SK ribonucleoprotein. (A) Immunoprecipitates from a HeLa extract with LARP7 (lane 1), CDK9 (lane 2), HEXIM1 (lane 3) antibodies and control serum (NRS; lane 4). Proteins were separated by SDS–polyacrylamide gel electrophoresis and bands marked with an asterisk were identified by mass spectrometry. The lower panel shows an ethidium bromide staining of the co-purified 7SK RNA. The abbreviation ‘hc' indicates heavy chains of the antibody, and ‘lc' indicates light chains of the antibody. (B) Mock-treated (lanes 1–7) or RNase A-treated (lanes 8–12) extracts were immunoprecipitated (IP) with the indicated antibodies. Immunoblots were probed with the indicated antibodies. 7SK RNA from the immunoprecipitates and the supernatants (Sup) are presented in the lower two rows. (C) Extracts derived from mock- or DRB-treated HeLa cells were immunoprecipitated with LARP7 and CDK9 antibodies. The indicated proteins were detected by western blot and 7SK RNA was visualized by ethidium bromide staining. CDK9, cyclin-dependent kinase 9; CYCT1, cyclin T1; DRB, 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole; HEXIM1, hexamethylene bis-acetamide inducible 1; LARP7, La-related protein 7; WB, western blot.