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. 2008 May 16;9(6):569–575. doi: 10.1038/embor.2008.72

Figure 5.

Figure 5

Knockdown of LARP7 enhances polymerase II transcription. (A) TAT-dependent transcription of a firefly luciferase reporter under the control of the HIV-1 long terminal repeat promoter in HeLa cells. The reporter is activated on transfection of a plasmid encoding the HIV-1 TAT protein (TATwt; bar 8), whereas mutant TAT (TATmut; bar 7) fails to activate (bars 9,10). The effect on reporter expression as a result of transfection of siRNAs against LARP7 (RNAi LARP7) is shown in bars 4–6 and that against CDK9 (RNAi CDK9) in bars 1–3. (B) Western blot of cellular extract treated with CDK9 (lanes 2,3) or LARP7 siRNA (lanes 4,5) and quantification of the signals normalized to HEXIM1, CDK9 and LARP7 (lane 1; lower panel). (C) Quantitative PCR levels of the indicated RNAs after RNAi treatment of LARP7 (dark bars) or HEXIM1 (light bars), normalized to 18S ribosomal RNA (18S). CDK9, cyclin-dependent kinase 9; HEXIM1, hexamethylene bis-acetamide inducible 1; LARP7, La-related protein 7; RNAi, RNA interference; siRNA, short interfering RNA.