Figure 2.

Fusion of a promoter of interest to mec-4(d) verifies promoter activity profiles and creates behavioral defects. (a) β-galactosidase staining of candidate ASH, OLQ, and IL1 neurons in animals harboring a p_del-2lacZ gene fusion. (b) Nomarski differential interference contrast microscopy image of vacuolated cells (indicated by white arrows) in a transgenic L2 animal harboring p_del-2mec-4(d). (c) Assay of nose touch responsiveness in transgenic animals harboring p_del-2mec-4(d). Transgenic lines also include pRF4, which encodes dominant rol-6(su1006). Dark gray bars indicate assay in the wild type background; light gray bars indicate assay in the mec-6(u450) background. Scores are the average of 100 animals per line, 10 trials per animal, for two independently derived transgenic lines. Error bars indicate the SD.