Figure 2.

Effect of IL-2, IL-7, and IL-15 on the accessibility of epitopes to FITC-conjugated mAbs 7G7/B6 (against IL-2Rα), Mikβ3 (against IL-2Rβ), and TUGh4 (against IL-2Rγ) added as single agents on Kit 225 K6 wild-type T cells. Interleukins were added to cell cultures 6 hr before harvesting at the following concentrations: IL-2 (solid bars), 20 units/ml; IL-7 (shaded bars) and IL-15 (open bars), both 1 ng/ml (20 pM). The mean values of fluorescence histograms collected on cells treated with the interleukins were expressed as percent change from control values. Bars represent mean ± SEM of four to six independent measurements.