Figure 4.

Relationship of ICAM-1 to NK lysis. (A) Expression of ICAM-1 (CD54) on NK-high sensitivity and NK-low sensitivity cell lines. The anti-CD54 mAb (Becton Dickinson) as well as its isotype matched control (mouse IgG_2b) were used. Fluorescein isothiocyanate-goat-anti-mouse IgG was used as a second antibody. (B) Lysis of NK-high sensitivity and NK-low sensitivity targets. Cells, non- or pretreated with the anti-CD54 mAb, were washed and incubated with NK lines 64 and 66 generated from healthy donors. Both NK lines manifested similar lysis patterns; the lysis by NK line 64 is shown. NK effectors were reacted with the indicated [³⁵S]methionine-labeled target cells for 5 h and the data obtained at an E/T of 10/1 are shown.