Table 1.
Spleen cells and peritoneal washes from 8- to 12-week-old mice were depleted of red blood cells and stained with the following markers: anti-B220 vs. anti-IgM; anti-IgM vs. anti-IgD; anti-CD4 vs. anti-CD8; anti-B220 vs. anti-CD5
| Cell type | xid | xid1xtg | xid2xtg | wt | wt1xtg | wt2xtg |
|---|---|---|---|---|---|---|
| Spleen | ||||||
| % B220+ | 36 ± 6.5* | 49 ± 6.2 | 48 ± 4.2 | 49 ± 5.4 | 51 ± 1.7 | 56 ± 4.3† |
| # B220+ (×106) | 26 ± 8.4* | 48 ± 22 | 37 ± 10 | 40 ± 12 | 47 ± 18 | 48 ± 17 |
| % IgMloIgDhi | 9.7 ± 2.7* | 31 ± 5.0 | 31 ± 2.5 | 34 ± 4.3 | 36 ± 4.8 | 38 ± 4.4† |
| # IgMloIgDhi (×106) | 7.0 ± 2.6* | 31 ± 17 | 24 ± 6.4 | 27 ± 9.0 | 34 ± 16 | 36 ± 13 |
| % CD4+ | 21 ± 3.6 | 21 ± 2.4 | 20 ± 2.0 | 20 ± 4.9 | 23 ± 1.5 | 21 ± 3.7 |
| % CD8+ | 12 ± 2.0† | 9.7 ± 1.6 | 10 ± 1.9 | 8.9 ± 2.4 | 10 ± 1.3 | 8.0 ± 1.9 |
| Peritoneum | ||||||
| % CD5+B220+ (all cells) | 1.6 ± 0.62* | 3.2 ± 2.0* | 5.7 ± 3.1* | 20 ± 7.6 | ND | ND |
| % CD5+B220+ (lymphoid cells) | 7.0 ± 2.4* | 11 ± 6.0* | 18 ± 9.0* | 39 ± 12 | ND | ND |
For spleen cells, live cells were gated based on forward and side scatter. For peritoneal cells, the gate was either set on live cells (all cells) or lymphoid cells (lymphoid cells) based on forward and side scatter. The percentage or number of cells having the indicated phenotype is presented as mean ± SD. Between 6 and 20 animals were analyzed per experiment. ND, not determined.
*
Decreased relative to wild type (P < 0.001).
†
Increased relative to wild type (P < 0.05).