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. 1997 Nov 25;94(24):13163–13168. doi: 10.1073/pnas.94.24.13163

Table 1.

TRFA measurements on DM in solution show that pH variation does not result in gross alterations of the DM conformation

pH D μs−1 θrms, ° Dsphere, μs−1
7.3 22.5  ±  2.6 20.7  ±  0.7 5.3  ±  0.7
5.0 18.2  ±  2.4 19.9  ±  0.7 4.6  ±  0.7

The diffusion coefficient D and the amplitude θrms are parameters calculated from the TRFA measurements to monitor the internal dynamics of the DM complex. The diffusion coefficient Dsphere was calculated as a parameter expressing the aggregation behavior of DM. All measurements were performed in duplicate.