Figure 4.

DRM2 and siRNA-dependent silencing of SDC transgenes. (A) Analysis of DNA methylation using sodium bisulfite sequencing of gSDC transgenes transformed by Agrobacterium into wild-type (Columbia) or drm1 drm2 backgrounds. Percentage methylation is shown for CG (black), CHG (gray), and CHH (white) sites. (B) Analysis of SDC expression by Northern blotting and hybridization in wild-type and mutant backgrounds transformed with gSDC transgenes. Loading was analyzed by hybridization with a UBIQUITIN10 (UBQ) probe. (C) Northern blot analysis of SDC expression in wild-type and mutant plants transformed with gSDC transgenes. (D) Northern blot analysis of SDC expression after drm1 drm2 mutants with unsilenced gSDC were backcrossed to wild type.