Confocal Immunofluorescence Microscopy Images Showing the Subcellular Localization of [FeFe] Hydrogenase in Blastocystis
The images show a Blastocystis cell with one nucleus and two mitochondrion-like organelles. (A) DAPI staining of the nucleus (bright) and mitochondrial DNA (faint; see arrows); (B) immunolocalization of [FeFe] hydrogenase using anti-BlastocystisHyd antibody; (C) MitoTracker orange staining of the mitochondrion-like organelles; (D) colocalization of MitoTracker orange, anti-BlastocystisHyd antibody, and DAPI in the mitochondrion-like organelles; and (E) DIC image of the Blastocystis cell.