Figure 2.

Analysis of the Organellar Genome Fragment

(A) Schematic representation of an ∼6 kb fragment from the Blastocystis organellar genome. Black boxes represent tRNA genes, which are identified by the amino acid they incorporate. nad3 and nad6 are NADH dehydrogenase subunits. M^e is the elongator methionine tRNA, and M^f is the initiator (formyl-) methionine tRNA. rns and rnl are the small and large subunit ribosomal RNA genes, respectively. Genes are not drawn according to scale.

(B) Maximum-likelihood tree derived from analysis of six concatenated mitochondrial genome-encoded proteins (Nad2, Nad3, Nad4, Nad6, Nad9, and Rps10). The clade containing eukaryotes is shaded. The analysis was based on 1137 unambiguously aligned positions. The tree was rooted arbitrarily with Dehalococcoides and Cyanobacteria. Numbers at nodes represent ML bootstrap values calculated with IQPNNI. Black dots on branches indicate bootstrap values ≥90%. Values below 50% are not shown.