Figure 2.

Reduced abundance of the IL-9 candidate in hyporesponsive B6 mice. (A) RNA were isolated from B6 and D2 splenocyte in vitro after 0, 12, 24, 48, and 72 h of Con A stimulation. Southern blots of RT-PCR for IL-9, IL-4, IL-5, and interferon-γ are shown. Actin RNA were used as an internal control. The symbols (+) and (−) designate with and without reverse transcriptase, respectively. (B) Western blot analysis of cytokines from the lungs of hyporesponsive B6, intermediate (B6D2)F₁, and hyperresponsive D2 mice demonstrating reduced IL-9 levels in B6 mice.