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. 1997 Nov 25;94(24):13221–13226. doi: 10.1073/pnas.94.24.13221

Figure 1.

Figure 1

(Left) Bandshift. Phosphorylation generates the different CUG-BP1 and CUG-BP2 isoforms. Bandshift assay of the CUG-BP1 and CUG-BP2 purified from HeLa cells in the presence of alkaline phosphatase. (Right) Western. CUG-BP/hNab50 Western blot analysis of the protein extracts prepared from HeLa cells treated with alkaline phosphatase. Whole cell protein extract (20 or 100 μg; lanes 1 and 2, respectively) was analyzed with mAb 3B1. Two isoforms, CUG-BP1 and CUG-BP2, are indicated. In phosphatase assay 100 μg of whole cell protein extract were used because affinity of mAb 3B1 is much higher to the hyperphosphorylated CUG-BP.