Figure 2.
Expression of CUG-BP isoforms in tissues from unique patient homozygous for DM mutation. Whole cell protein extracts were prepared from tissues of patient with DM (A) or cardiac tissue from normal control and DCM patient (B) and analyzed by bandshift assay with RNA (CUG)8 probe. Positions of CUG-BP1, CUG-BP2, and ssCRRP (13) are indicated. Specificity of binding was examined by addition of 100 ng of unlabeled (CUG)8 RNA oligonucleotide. (C) CUG-BP is accumulating in nuclei in cultured cells from DM patients. Cytoplasmic and nuclear protein extracts were subjected to SDS/PAGE and immunoblotted with antibody 3B1. Reprobing of the same membrane with antibodies to cdk4 or anti-β-actin (Lower) shows equal loading of proteins.