Figure 3.
CUG-BP/hNab50 expression in skeletal muscle and heart tissue from DMPK knockout mice and genetically normal littermates. (A) Western blot analysis of cytoplasmic and nuclear protein extracts with antibody 3B1. CUG-BP/hNab50 is translocated to nuclei in DMPK null mutants. (B) CUG-BP2 binding activity is increased in nuclear extracts from cardiac tissue of DMPK null animals. Cytoplasmic and nuclear proteins from cardiac tissue of DMPK knockout and genetically normal littermates were analyzed by UV-cross link with RNA (CUG)8 probe as described in Materials and Methods. The same membrane was stained with Coomassie Blue showing equal protein loading. The positions of molecular mass markers are shown (Left).