Table 1.

P. aeruginosa mutants obtained by TnphoA mutagenesis

Strain	Growth in Arabidopsis leaves*	Symptoms elicited in Arabidopsis†	% mouse mortality‡		Gene identity
			5 × 103	5 × 105
PA14	5.5  ×  107	Severe	53	100
33C7	8.3  ×  104	None	0	0	Unknown§
1D7	7.5  ×  105	Weak	0	50	gacA
25A12	1.7  ×  106	Weak	11	87	Unknown
33A9	5.1  ×  106	Moderate	0	0	Unknown
25F1	1.5  ×  104	Moderate	0	20	Unknown
34H4	3.8  ×  106	Moderate	0	33	Unknown
pho34B12	4.0  ×  106	Moderate	0	56	Unknown
pho15	3.9  ×  104	Moderate	0	62	dsbA
16G12	2.3  ×  105	Moderate	20	100	Unknown

^*Four different samples were taken using two leaf discs/sample. Control plants inoculated with 10 mM MgSO₄ showed no symptoms during the course of the experiments. Three independent experiments gave similar results. 

^†Symptoms observed 4–5 days after infection. None, no symptoms; chlorosis, chlorosis circumscribing the inoculation site; weak, localized water-soaking and chlorosis of tissue circumscribing the inoculation site; moderate, moderate water-soaking and chlorosis with most of the tissue softened around the inoculation site; severe, severe soft-rotting of the entire leaf characterized by a water-soaked reaction zone and chlorosis around the inoculation site at 2–3 days postinfection. 

^‡All animal experiments were conducted at least twice using 8–10 animals/experiment. Independent experiments showed similar percentage mortality rates. Mice were injected with ≈ 5 × 10³ or 5 × 10⁵ cells. 

^§blastx analysis yielded no encoded proteins with significant homology.