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. 1997 Nov 25;94(24):13257–13261. doi: 10.1073/pnas.94.24.13257

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Copyright © 1997, The National Academy of Sciences of the USA

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Interaction between CooB and CooC. Periplasmic extracts (A and C, lanes 1–4) and whole membranes (B and D, lanes 1–4) were separated on SDS/PAGE and probed with antibodies to CooB (A and B) or maltose binding protein (C and D). (Left) Molecular masses (in kDa) determined by a lane containing markers. Lane 1, MC4100/pFDX500/pHSG576/pUC19 (Coo⁻). Lane 2, MC4100/pFDX500/pEU479/pNEBCS1.3 (CooCCooB). Lane 3, MC4100/pFDX500/pEU479/pUC19 (CooC). Lane 4, MC4100/pFDX500/pHSG576/pNEBCS1.3 (CooB).