Figure 3.—

argos and rho expression is regulated by InR/TOR signaling in developing neurons. (A and A′) The level of Argos protein (detected using an anti-Argos monoclonal antibody, shown in red) is increased in pten¹ mutant cells (marked by loss of GFP staining). Note how Argos staining is seen ahead of the normal expression front (marked with an arrow). (B and C) argos expression is regulated by InR/TOR signaling at the level of transcription. Expression of β-galactosidase (stained with an anti-β-galactosidase antibody, shown in red) from the P{lwB}-element insertion in argos (aos^w11) is upregulated in pten¹ clones (B and B′) and downregulated in Rheb^2D1 clones (C and C′). (D and D′) rho expression (using the rho^X81 reporter, detected by staining with a anti-β-galactosidase antibody, shown in red) is upregulated in pten¹ clones. Clones are marked by loss of GFP staining and anterior is to the left in all panels.