Figure 3.—

Western blot analysis of TPI protein levels. (A) TPI^sugarkill mutants show a reduced level of TPI protein when aged for 48 hr at room temperature and 29° compared to actin controls. (B) Quantification of Western blot shows an ∼3.5-fold decrease in TPI protein levels at room temperature and an ∼48-fold decrease at 29°. *P < 0.05 (Student's t-test). Error reported is SEM. N = 3 at each temperature. Intensity of TPI was measured from a subsaturation blot and normalized to the intensity of the actin control using ImageJ 10.2.