Figure 5.—

Clb2 requires N and K for interaction with Swe1. Unsynchronized GAL-SWE1-MYC6 cells expressing CLB2 (YGy72) or CLB2-ME (YGy69) from the endogenous CLB2 promoter were incubated in YEP/2% raffinose (Raf) or YEP/2% galactose (Gal) for 2 hr at 30°. (A) Equal amounts of soluble proteins (Extract) were subjected to immunoprecipitation (IP) with anti-Myc antibody, followed by immunoblot analysis of the precipitated proteins with anti-Myc and anti-Clb2 antibodies. (B) Quantification of the amounts of Clb2 and Clb2-ME relative to the amounts of precipitated Swe1. The values are based on chemiluminescent signal intensities and are dimensionless; error bars indicate standard deviation (n = 2).