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ClustalW alignment (http://www.ebi.ac.uk/clustalw) showing polymorphisms between the female and male allelic sequences of the sex-specific AFLP marker E06M45M347.0 (solid background). The EcoRI and MseI sites are shaded; primers ATT/GCA-1 and ATT/GCA-2, used for amplifying both the female and male fragments from genomic DNA, are underlined; primers indel-4 and indel-5, flanking the 6-bp indel polymorphism, are boxed.
