TABLE 5.

Expected and observed segregation patterns for sex-linked AFLP markers under the assumption of either a WZ–ZZ or a XX–XY sex-determination system

					No. of observations
Patterna	Dam genotype	Sire genotype	Genotype of female offspring	Genotype of male offspring	FAM1	FAM2	FAM3
			WZ–ZZ
1	Aa	aa	Aa	aa	7	7	10
2	aA	aa	aa	Aa	1	0	3
3	aA	AA	aA	AA	0	0	0
4	aA	aA	Aa or aa	AA or Aa	NI	NI	NI
5	aa	Aa	Aa or aa	Aa or aa	NI	NI	NI
			XX–XY
6	aa	aA	aa	Aa	0	0	0
7	aa	Aa	Aa	aa	0	0	0
8	AA	Aa	AA	Aa	0	0	0
9	Aa	Aa	AA or Aa	Aa or aa	NI	NI	NI
10	Aa	aa	Aa or aa	Aa or aa	NI	NI	NI

WZ–ZZ, patterns 1–5; XX–XY, patterns 6–10. The A allele represents the “AFLP band present” allele. NI, not informative.

^aOffspring genotype classes as observed for segregation patterns 4 and 9 cannot unambiguously discriminate between the two sex-determination mechanisms. Offspring genotype classes observed for patterns 5 and 10 are identical to those expected for non-sex-linked markers. Hence, observations of these four patterns are not informative in characterizing the sex-determination system. Segregation patterns unique for the two sex-determination mechanisms are in italics.