Figure 5.

Effect of paxillin-siRNA on the formation of experimental metastases. A. Immunoblot showing that paxillin expression was reduced in B16-F10 cells transfected with paxillin-siRNA (pax). Densitometric quantification (graph) show that the decrements on paxillin/tubulin ratios (mean ± SE, n = 3) in cells transfected with paxillin-siRNA (open bars) were similar to those produced by 4-HC. B. The participation of paxillin in metastasis formation was studied by injecting into mice cells transfected with either paxillin- or control-siRNA. Paxillin downregulation produced a 1.6-fold reduction in the number of experimental pulmonary metastases. * P = 0.04 (Student's t test). The pictures on the right show examples of lungs from mice injected with siRNAs-trated cells.