Figure 4.

Effects of NO-induced incorporation of NAD into G3PD on subsequent binding of the G3PD to actin. Analyses of binding of G3PD to actin was performed by using the centrifugation approach, followed by SDS/PAGE as described. Lanes 1 and 2 are standards without incubation. Lanes 3 and 4 are pellet (P) and supernatant (S) of actin incubated with G3PD but without preincubation. Lanes 5 (P) and 6 (S) are G3PD preincubated with SNP and NAD. Lanes 7–10 are controls in the absence of NAD (lanes 7 and 8) or in the absence of SNP (lanes 9 and 10). Proteins are stained with Coomassie brilliant blue. The left lane contains molecular weight standards.