Figure 7.

(A) Effects of exogenous NO-stimulated NAD incorporation (Exo-NOSNI) of PSD proteins on subsequent formation of the endogenous ATP. Experiments were performed as described. PSD fractions (100 μg aliquots) pretreated with (lanes 3 and 4) and without (lanes 1, 2, 5, and 6) SNP plus NAD were subsequently incubated at room temperature for 10 min with ³²Pi in the absence (lanes 1 and 2) and presence (lanes 3–6) of ADP. The mixtures were examined for phosphorylation with (lanes 2, 4, and 6) or without (lanes 1, 3, and 5) Ca²⁺/CaM and were then subjected to SDS/PAGE for autoradiographic analysis. (B) Effects of endogenous NO-stimulated NAD incorporation (Endo-NOSNI) of PSD proteins on subsequent formation of the endogenous ATP. PSD samples (100 μg aliquots) preincubated with (lanes 3 and 4) or without (lanes 1, 2, 5, and 6) NAD, 0.5 mM l-arginine, 1 mM each of FMN/FAD, and 2 mM Ca²⁺/0.25 mM CaM were subsequently incubated at room temperature for 10 min with ³²Pi in the presence (lanes 3–6) or absence (lanes 1 and 2) of ADP. The reaction mixtures were then processed for phosphorylation in the absence (lanes 1, 3, and 5) or presence (lanes 2, 4, and 6) of Ca²⁺/CaM.