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. 2007 Dec 18;4(2):176–178. doi: 10.1098/rsbl.2007.0583

Figure 1.

Figure 1

Agarose gel showing identification of chromosomal sex for two females and two males. Upper half of gel: duplex PCR amplification of 356 bp C-mos fragment (males and females) and 185 bp Y-chromosome fragment (males only) from genomic DNA extracted by high-salt method. Lower half of gel: duplex PCR amplification of 356 bp C-mos fragment (females only) and 92 bp Y-chromosome fragment (males only) from genomic DNA extracted by Chelex method. The Y-chromosome fragment is amplified preferentially over the positive-control C-mos fragment for the Chelex-extracted DNA. Lane 1 shows molecular weight marker.