Figure 3. Characterization of VSFP3.1 ‘gating’ currents and fluorescence response.

Representative ‘gating’ currents (a) and fluorescence responses (b) of VSFP3.1 elicited from single cells upon a family of voltage steps. (c) Voltage-dependency of the fast component of the fluorescence activation time-constant (blue) and the time-constant for the decay of on-‘gating’ currents (black). The inset shows single exponential fits to the fast fluorescence time-constant component from averaged fluorescence traces (n = 8) obtained with voltage steps to −30 mV and +70 mV.