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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: Dev Biol. 2008 Mar 12;317(2):541–548. doi: 10.1016/j.ydbio.2008.02.051

Figure 4.

Figure 4

Cx43 protein expression is reduced in sofb123 mutants. (A) An antibody raised against zebrafish Cx43 detects a GST fusion protein with the carboxy tail of Cx43 (GST-Cx43CT). Increasing amounts of E. coli lysates expressing GST-Cx43CT were loaded and test for antibody detection (left). When the antibody was pre-incubated with the Cx43 target sequence, binding to the immunoblot was competed. (B) Protein levels of Cx43 in sofb123 regenerating fins and whole body are reduced by greater than 70 %. Tubulin was used as a loading control and to normalize the loading of fin or whole body lysates. (C) Percent reductions of anti-Cx43 antibody binding for the antibody competition (A) and sofb123 Cx43 levels (B). (D) Cx43 expression in wild-type fins. Distal tip staining in wild-type (E) and sofb123 fins (F). (G) Cx43 expression is substantially reduced in sofb123 mutant fins. Arrows point to Cx43 staining at the joints.