Figure 2.

Neurotrophins induce BDNF release from acute hippocampal slices. Time course of BDNF release from hippocampal slices before, during, and after application of 50 μM glutamate or 100 ng/ml neurotrophins (NT-4/5, NT-3, or NGF). Fractions were collected every 5 min, and BDNF contents were measured with a two-site ELISA. A recovery phase of 30 min was allowed between different stimulations. The 5-min delay between the onset of stimulation and the peak of BDNF secretion is caused by the dead volume of the perfusion system (approximately 0.5 ml) corresponding to 5 min of perfusion time. Standard errors are referred to four independent experiments.