Figure 3.
Neurotrophins induce BDNF release from hippocampal neuron in culture. (A) Time course of BDNF release from hippocampal primary culture infected with an adenovirus expressing BDNF before, during, and after application of 50 μM glutamate or 100 ng/ml neurotrophins (NT-4/5, NT-3, and NGF). Hippocampal neurons in culture (3 × 105) overexpressing BDNF were perfused as described in Fig. 2. Standard errors are referred to four independent experiments. (B) Time course of glutamate recovered from hippocampal cultures. Aliquots of each fraction were analyzed for glutamate contents. The same set of experiments as that used to study the effect of NT-4/5. The dashed lines indicate the time of 50 μM glutamate application. (C) Cell viability was evaluated by labeling living (green) and dead (red) cells by using fluorescein-diacetate and propidium iodide before and after each release experiment.