Figure 4.
(A) BDNF induces BDNF-myc release from hippocampal neurons in culture. Release experiments were conducted under conditions without constant perfusion, “static conditions” for which 2 × 106/1.8 cm2 hippocampal neurons had been transduced by AdCMV-BDNF-myc. Release of BDNF-myc was induced either by 50 μg/ml glutamate or 100 ng/ml BDNF, and BDNF-myc content was determined by a two-site immuno-assay. (B) NGF release from hippocampal neuron in culture. Hippocampal neurons (3 × 105) were perfused continuously, and NGF release from cultured hippocampal neurons infected with AdCMV-NGF was induced either by 50 μM glutamate or 100 ng/ml neurotrophins (NT-4/5, NT-3, and BDNF). Standard errors are referred to four independent experiments.