Figure 8.

NGF induces BDNF release from PC12 cells. (A) Time course of BDNF release from undifferentiated PC12 cells before, during, and after application of 50 mM KCl or 100 ng/ml NGF. PC12 cells (3 × 10⁵) infected with an adenovirus gene transfer system expressing BDNF were perfused as described in Fig. 2. BDNF contents, expressed in pg/ml, was measured with a two-site ELISA system. (B) Release experiments were conducted under conditions without constant perfusion, “static condition” by using 2 × 10⁶/1.8 cm² confluent PC12 cells. Cells were infected with about 5 × 10⁶ plaque-forming units of an adenovirus gene transfer system expressing BDNF. After washing, the release of BDNF was initiated either by 50 mM KCl or 100 ng/ml neurotrophins (NGF, NT-4/5 or, NT-3) and BDNF content was determined by ELISA. Standard errors are referred to four independent experiments.