Fig. 4. Effect of silenced survivin expression on TRAIL-induced apoptosis in DU-145 cells.
Cells were transfected with a control vector or silenced survivin oligonucleotide DNA for 24 h. Transfected cells were treated for 4 h with various concentrations of TRAIL (10-200 ng/ml). (A) Cell lysates were subjected to immunoblotting for anti-PARP or anti-survivin. Actin was shown as an internal standard. (B) Cell survival was determined by the trypan blue exclusion assay. Error bars represent standard error from the mean (SEM) for three separate experiments.