. Author manuscript; available in PMC: 2008 Jun 16.

Published in final edited form as: Toxicol Sci. 2007 Jan 4;96(2):321–326. doi: 10.1093/toxsci/kfl200

TABLE 1.

Choline Metabolites in Neural Precursor Cells

	Choline (nmol/mg DNA)	Glycerophosphocholine (nmol/mg DNA)	Phosphocholine (nmol/mg DNA)	Phosphatidylcholine (nmol/mg DNA)	Sphingomyelin (nmol/mg DNA)
CT	43.5 ± 3.5	17.5 ± 2.7	255.0 ± 5.7	1186.8 ± 44.9	173.3 ± 17.4
CS	57.8 ± 1.4^a	16.0 ± 0.9	312.3 ± 11.2^a	1155.7 ± 9.0	166.0 ± 2.3
CT-DEA	24.1 ± 0.8^a,^b	14.2 ± 0.5	31.6 ± 0.5^a,^b	1175.0 ± 16.1	202.2 ± 6.2
CS-DEA	43.7 ± 0.4^b,^c	14.9 ± 1.2	74.6 ± 6.2^a,^b,^c	1229.5 ± 10.5	173.4 ± 2.4

Note. Neural precursor cells were cultured in Neurobasal medium (see the “Materials and Methods” section) containing 70μM choline (CT), 70μM choline, and 3mM DEA (CT-DEA); 210μM choline (CS); or 210μM choline and 3mM DEA (CS-DEA). Choline metabolites were measured by LC-MS (see the “Materials and Methods” section), after 48 h treatment. No betaine was detected in any sample. Superscripts denote statistical significance at p < 0.05;

Different than CT

Different than CS

Different than CT-DEA.