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. 2008 Jul;56(7):687–696. doi: 10.1369/jhc.2008.951194

Figure 5.

Figure 5

LM 3A32 immunohistochemistry and in situ hybridization in normal acute incisional wounds of 1 day (A,E,I), 3 days (B,F,J), 7 days (C,G,K), and ulcer (D,H,L). Immunostaining with LM 3A32 antibody shows staining of the 1-, 3-, and 7-day acute incisional wounds (A–C) but not in the ulcer (D). LM 3A32 mRNA using a digoxigenin-labeled LM 3A32 RNA antisense probe is present on the migrating epithelia of 1-, 3-, and 7-day acute incisional wounds (E–G) and not present in the ulcer margin tissue from a patient with diabetes (H). Arrows indicate presence of LM 3A325 mRNA. In situ hybridization using sense probes (I–L) served as controls. Dotted line indicates DEJ and star indicates ulcer margin.