Figure 2.

Angiosarcomas are also devoid of decorin. ICC and ISH analyses of serial sections of a representative specimen of angiosarcoma. (A) HE staining. (B) Staining with an antibody to the endothelial cell marker CD31. (C) ISH for human decorin. (D) Distribution of decorin identified with LF-136 antiserum for human decorin. (E) Distribution of type I collagen identified with LF-67 antiserum for human type I collagen. ICC reactions are brown and counterstain for nuclei by hematoxylin is blue. Positive DIG reaction in ISH assay can be seen in purple. Arrowheads (B–E) indicate the border between the angiosarcoma and its surrounding tissue. Asterisks in C show an area positive for decorin mRNA expression around the tumor. Asterisks in D,E show the location of positive immunoreactivity for decorin and type I collagen. Bar = 200 μm.