Table 1.
Spectral properties of selected lipophilic neurotracing dyes.
| Dye | Absorption maximum* (nm) | Extinction Coefficient (M−1cm−1)* | Extinction maximum (nm)** | Emission maximum**(nm) |
|---|---|---|---|---|
| Red/Far Red dyes | ||||
| NV Red | 565 | 100,533±5729 | 567 | 588 |
| NV Maroon | 650 | 216,700±907 | 647 | 667 |
| Green dyes | ||||
| DiA | 492*** | 49,000±4000 | 492 | 608 |
| DiO | 485*** | 153,000±7000 | 485 | 500 |
| NV Emerald | 492 | 132,400−134,610 | 494 | 507 |
| PTIR 326 | 490 | 75,800 | 492 | 520 |
| PTIR 327 | 450 | 79,400 | 449 | 473 |
| NV Jade | 478 | 78,083±8467 | 478 | 508 |
*
Determined in ethanol using a Jasco V530 UV-Vis spectrophotometer; extinction coefficients at absorption maxima as determined from the slope of a plot of absorbance vs. concentration using 5 dye concentrations in the range of 0.5–10 μM.
**
Obtained for 0.5–1.0 μM ethanolic solutions of dye using a Horiba Fluoromax-3 spectrofluorometer.
***
From Certificate of Analysis provided by Molecular Probes/Invitrogen.