Table 2.
Confocal and epifluorescence optics used for imaging selected lipophilic neurotracing dyes.
| Confocal Optics | ||||
|---|---|---|---|---|
| Dye | Excitation (nm) | Dichroic | Emission Filter Set | Epifluorescence Filter Set |
| Red/Far Red Dyes | ||||
| NV Red | 543 | NFT 545 | 565–615 nm | Texas Red |
| NV Maroon | 633 | NFT 490 | 650–710 nm | Cy5 |
| Green Dyes | ||||
| DiA | 488 | NFT 490 | 500–550 nm | Texas Red |
| DiO | 488 | HFT 488 | 500–550 nm | FITC |
| NV Emerald | 488 | HFT 488 | 500–550 nm | FITC |
| PTIR 326 | 488 | NFT 490 | 500–550 nm | FITC |
| PTIR 327 | 458 | HFT 458 | 480–520 nm | FITC |
| NV Jade | 488 | NFT 490 | 500–500 nm | FITC |