Table 4.
Relative and absolute detection distances for selected lipophilic neurotracing dyes in E18.5/P0 murine spinal Cord.
| Test Dye | Test Dye loading (nmole/mm2) | NV Red loading (nmole/mm2) | Time at 37°C | n | RDD, % (± SE) | Max. Detection Distance, Test Dye (mm±SE) | Max. Detection Distance, NV Red (mm±SE) |
|---|---|---|---|---|---|---|---|
| NV Maroon | 8 | 8 | 24h | 2 | 100 ± 0 | 1150 ± 100 | 1150 ± 100 |
| 96h | 2 | 100 ± 0 | 2838 ± 395 | 2838 ± 395 | |||
| NV Jade | 5.8 | 5.6 | 24h | 4 | 94 ± 3.5 | 1633 ± 33 | 1683 ± 17 |
| 48h | 4 | 96 ± 5.5 | 2291 ± 110 | 2300 ± 50 | |||
| 96h | 3 | 92 ± 0.5 | 1916 ± 148 | 2075 ± 170 | |||
| NV Jade | 10.1 | 10.1 | 24h | 4 | 104 ± 9.3 | 1375 ± 156 | 1366 ± 33 |
| 48h | 4 | 108 ± 2.3 | 1950 ± 126 | 1808 ± 102 | |||
| 96h | 4 | 106 ± 3.4 | 1875 ± 111 | 1775 ± 118 | |||
| PTIR 326 | 5.5 | 6 | 24h | 3 | 104 ± 4.8 | 1708 ± 262 | 1658 ± 287 |
| 48h | 6 | 104 ± 3.0 | 1725 ± 149 | 1658 ± 125 | |||
| 96h | 3 | 102 ± 1.6 | 1837 ± 114 | 1612 ± 185 | |||
| PTIR 326 | 12.3 | 11.6 | 24h | 5 | 106 ± 3.1 | 1254 ± 90 | 1170 ± 58 |
| 48h | 5 | 114 ± 4.9 | 2450 ± 93 | 2131 ± 62 | |||
| 96h | 4 | 95 ± 3.1 | 2125 ± 298 | 2237 ± 272 | |||
| PTIR 327 | 34.7 | 24 | 24h | 2 | 114 ± 14 | 1325 ± 100 | 1150 ± 100 |
| 48h | 3 | 104 ± 14 | 2025 ± 0 | 1675 ± 76 | |||
| 96h | 1* | 0* | 0* | 2100* | |||
| DiA | 5.6 | 6.1 | 24h | 2 | 124 ± 6.6 | 1600 ± 100 | 1300 ± 150 |
| 48h | 2 | 107 ± 2.3 | 1950 ± 300 | 1825 ± 275 | |||
| 96h | 2 | 103 ± 0.5 | 2175 ± 75 | 2125 ± 125 |
*
Representative specimen (one of 6 in 3 independent studies); others in group not measured.