Table 2.

Clinicopathological characteristics, LOH and gene expression variation in folds* for 16 tumor samples.

Sample (Patient) no.	Age (yrs)/Sex	Tumor classification	Metastasis	Tobacco use	LOH@ TSC1	LOH@ TSC2	TSC1 (D)	TSC2 (D)	AKT1 (U)	PIK3C2A (U)	PDPK1 (U)	RHEB (U)	FRAP1 (U)	RPS6KB1 (U)	RPS6 (U)	EIF4E (U)	EIF4EBP1(D)	PTEN (D)
54	40 M	T4N1M0	N/A	Yes	-	-	1.39	2.04	2.04	2.34	0.77	2.29	1.82	2.25	3.40	2.23	3.12	1.70
39	55 F	T4N1M0	Yes	Yes	-	-	1.77	1.95	4.04	2.47	2.33	1.92	2.68	3.34	2.67	3.01	0.98	1.44
19	48 F	T4N2bM0	No	Yes	-	-	1.93	3.57	3.29	1.63	1.57	2.12	2.21	2.93	2.01	2.07	1.45	2.04
15	55 F	T3N1M0	No	Yes	-	-	2.63	2.03	4.68	3.23	1.81	0.76	1.76	1.48	2.83	1.31	3.03	2.35
20	50 F	T4N1M0	Yes	Yes	-	-	1.98	2.03	1.46	1.65	0.65	0.85	0.92	1.34	1.50	1.98	3.02	1.87
8	50 F	T3N1M0	No	Yes	-	-	1.15	1.37	1.57	0.95	1.39	1.73	0.44	0.61	0.64	1.54	4.27	3.67
11	60 F	T4N1Mx	Yes	Yes	-	-	0.31	0.69	1.80	1.69	1.03	2.54	3.76	1.94	0.69	1.17	0.96	2.36
40	50 F	T2N1M0	Yes	Yes	+	-	2.33	1.48	1.75	2.16	1.90	2.22	2.82	6.25	1.83	4.22	2.06	1.60
50	40 M	T4N2bM0	Yes	Yes	-	-	1.20	2.98	1.34	0.58	1.43	1.22	1.25	2.45	2.69	1.50	3.67	1.35
52	70 M	ED	N/A	No	-	-	2.35	1.82	2.69	2.64	1.50	2.23	5.78	1.80	1.76	3.74	1.69	2.23
32	35 M	T4N0Mx	No	Yes	-	-	1.58	3.03	2.04	0.90	1.15	0.80	1.48	1.67	1.89	1.60	2.24	1.18
53	38 F	T2N0M0	No	Yes	+	-	7.92	1.51	3.68	2.88	2.59	2.78	1.19	2.32	1.21	2.13	2.77	1.99
55	40 F	T4N1M0	Yes	Yes	+	-	1.35	1.81	2.27	2.99	1.53	1.70	12.4	1.37	4.47	2.67	1.77	1.46
57	62 F	T1N0M0	Yes	Yes	-	-	1.40	2.39	2.26	4.50	8.26	2.95	2.24	5.05	1.80	2.32	2.17	0.50
59	70 F	T4N2bM0	Yes	Yes	-	+	2.38	1.30	1.48	2.80	1.92	1.67	1.38	0.67	1.66	1.70	5.07	1.23
60	40 F	T4aN1Mx	Yes	Yes	-	-	1.37	1.80	0.87	2.28	1.49	1.98	1.05	1.04	1.99	1.88	0.60	1.30

* We defined the cutoff value for determining the upregulation or downregulation of a gene in a tumor sample as ≥ 1.8 fold difference in its expression between normal and tumor samples [3]. With this criterion, TSC1, TSC2, EIF4EBP1 and PTEN genes showed downregulation in 7/16, 11/16, 10/16 and 7/16 tumor samples, respectively. Whereas AKT1, PIK3C2A, PDPK1, RHEB, FRAP1, RPS6KB1, RPS6 and EIF4E showed upregulation in 10/16, 10/16, 6/16, 9/16, 8/16, 9/16, 10/16 and 10/16 tumor samples, respectively.

Abbreviations: TNM, Tumor, Node, Metastasis; and ED, Epithelial dysplasia.

'Yes' in tobacco use refers to addiction to tobacco, bidi and cigarettes for at least 15–20 years. '+' denotes LOH found; '-' denotes no LOH; N/A, not applicable;

(D)denotes downregulation of gene expression and (U)denotes upregulation of gene expression across 16 tumor samples.