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. Author manuscript; available in PMC: 2009 May 2.
Published in final edited form as: Vaccine. 2008 Mar 31;26(19):2335–2343. doi: 10.1016/j.vaccine.2008.02.064

Figure 7. Immunization of dogs with rH_EIV results in a reduction in amount and duration of viral shedding upon challenge with virulent CIV.

Figure 7

Two groups of four dogs were vaccinated s.c. twice with rH_EIV or mock-vaccinated. All dogs were challenged 3 weeks after booster vaccination with 1×106 PFU A/canine/PA/10915-07 and nasal swabs were taken on days 0–8, 10, and 15. RT-PCR was used to determine virus titers based on the number of influenza RNA copies (A). Virus isolation was performed by inoculating swab fluids into MDCK cells (B) and the presence or absence of virus was also determined by inoculation of eggs (C). Asterisks indicate statistically significant differences.