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. 2008 Jun 3;105(23):8067–8072. doi: 10.1073/pnas.0709684105

Fig. 1.

Fig. 1.

Kinetics of production and secretion of IL-1β in response to different PAMPs and DAMPs. Monocytes were cultured for different periods of time in the absence (CTR) or presence of LPS, MDP, zymosan (ZYM), S. aureus (STAPH A), flagellin (FLAG), or MSU, alone or in association as indicated. (A) Western blot of IL-1β in cell lysates at 3 or 15 h of exposure to stimuli. Migration of the 35-kDa pro-IL-1β molecular form is indicated. (B and C) ELISA of IL-1β released by monocytes cultured for 3, 6, or 15 h with the same PAMPs or DAMPs as in A. Note that LPS+ATP represents the amount of IL-1β released during 15 min of exposure of LPS-treated monocytes to exogenous ATP. (D) ELISA of IL-1β released by monocytes cultured for 6 h with PAMPs or DAMPs (empty columns) followed by 15 min of ATP (filled columns). Results are expressed as ng/ml/106 cells. Values are the mean ± SEM of five experiments performed on monocytes from different donors.