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. 2008 Jun 20;118(7):2609–2619. doi: 10.1172/JCI34588

Figure 1. A431 GR cells maintain PI3K/Akt signaling in the presence of gefitinib and remain sensitive to PI3K inhibitors.

Figure 1

(A) Parental and A431 GR cells were grown for 72 hours in 0.5% FBS containing medium with or without gefitinib at the indicated μM concentrations. Cell number was determined in a Coulter Counter. Each data point represents the mean ± SD of 3 wells. (B) Parental and A431 GR cells were grown in Matrigel in the absence or presence of gefitinib (1 μM). Pictures were taken after 10 days. Original magnification, ×10. (C) Parental and A431 GR cells were treated for 6 hours in growth medium containing 1 μM gefitinib. Whole-cell lysates were prepared and probed in immunoblots with the indicated antibodies. (D) Parental and GR cells were treated with control, gefitinib (3 μM), or erlotinib (3 μM), and the extracts were probed with the indicated antibodies. (E) Cell growth assays were performed as in A. Cells were treated with the indicated drugs and concentrations. (Gef, gefitinib; Erlot, erlotinib; LY, LY294002). Student’s t test was used for statistical comparisons. Error bars represent SD.