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. Author manuscript; available in PMC: 2008 Jun 17.
Published in final edited form as: Cornea. 2006 Oct;25(9):1063–1068. doi: 10.1097/01.ico.0000228785.84581.35

Figure 2.

Figure 2

Hyperosmolar medium does not induce hBD-2 expression in HCECs. A, SV40-HCECs (n = 4) were cultured for 24 hours in serum-free normal osmolality media only (N) or with the addition of IL-1β as a positive control (IL-1β) or serum-free hyperosmolar media. B, The cells were exposed to 500 mOsm/kg (NaCl) media for various lengths of time or to IL-1β (positive control) or normal media for 24 hours. The figure shows RT-PCR products for GAPDH and hBD-2. Similar results were obtained when the experiment was repeated with P-HCECs (n = 2; data not shown). M, size marker.