Figure 6. Strategy for Effective and Rational Coverage of the MHC Polymorphism and Specificity.

(A) The pan-specific MHC class II prediction method is used to identify MHC alleles with novel binding specificities. These alleles have a predicted binding motif that is distant to all MHC class II molecules previously described. Subsequently, immunoassays are developed describing their binding specificity and data is fed back into a retraining of the pan-specific method. (B) Next, peptides with un-characterized binding affinity (high information peptides) are identifies, experimentally assayed and fed back into the retraining.