Figure 3.

NMR Relaxation Measurements Reveal Rigidification of LaNTD upon RNA Binding

The R₂/R₁ ratios for apo-LaNTD (open circles) and the LaNTD:UUUU complex (closed circles) are plotted against amino acid number. Also shown are HYDRONMR-predicted ratios for RNA-bound LaNTD (medium gray line) and the individual domains, the La motif (light gray line) and RRM1 (black line). Relaxation rates were calculated from peak intensity decay curves fit using CurveFit (http://cpmcnet.columbia.edu/dept/gsas/biochem/labs/palmer/). Uncertainties were calculated using jackknife simulations (Mosteller and Tukey, 1977).