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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Hear Res. 2008 Apr 3;240(1-2):1–11. doi: 10.1016/j.heares.2008.03.003

Fig. 4.

Fig. 4

Confocal images of cochleae labeled for activated caspase-3 (green) using a CaspaTag in situ assay kit 30, 42, 48, 72, and 96 h AI. Early time points (30, 42, and 48 h) are co-labeled with phalloidin (red); later time points (72 and 96 h) are co-labeled with myosin VI (red). Overlap of the two labels appears in yellow. The timing of caspase-3 activation using CaspaTag mirrors that seen with caspase-directed antibodies, with labeling first occurring between 30 and 42 h AI. However, the CaspaTag assay appears to label more cells at the later time points, such as 72 and 96 h. Scale bar = 300 μm.