Fig. (11).

Experiments supporting the view that PGE₁ acts on NG108-15 cells via increase of intracellular cAMP and Ca²⁺. A-C: Pen records of holding current recorded at -70 mV. A: Effect of 0.5 mM CPT-cAMP. B: Effect of 1 μM thapsigargin. C: Effect of 0.2 μM PGE₁ in Ca²⁺- and Mg²⁺-free bath with 1.1 mM EGTA. D: Scatter plot showing the effect of 3 μM PGE₁ on holding current with 3 mM EGTA (n=18) or 20 mM BAPTA (n=7) in pipette solution; mean values indicated by horizontal bars. Perforated patch in A-C, ruptured patch in D. Room temperature. From [70, 71] and Meves (unpublished).