Table 2.

Estimated flux ratios from GC-MS measurements of B. subtilis.

flux ratios	our fw (UL)	our fw (1CL)	FiatFlux (UL)	FiatFlux (1CL)
PYR from MAL : PYR from PEP	0.01 : 0.99		0.04 : 0.96
OAA from TCA-cycle : OAA from PYR	0.42 : 0.58	0.37 : 0.63	0.41 : 0.59
PEP from OAA : PEP from GA3P	0 : 1	0 : 1	0.04 :0.96	0 : 1
GLY from SER : GLY from C1 + CO2	1 : 0	1 : 0	1 : 0
SER from GLY : SER from GA3P	0.09 : 0.91		0.14 : 0.86

Directly comparable flux ratios computed from the GC-MS data described by the framework presented in this paper (our fw) and by FiatFlux software [30]. UL denotes uniform labelling of external glucose, 1CL external glucose labelled to the first carbon position.